Scientist-B
CSB-Satellite Silkworm Breeding Station (SSBS), Cornwall Road, Coonoor -643101, Nilgiris District
Sericulture
My M.Sc. thesis work on “Management of mulberry root rot disease through Plant Growth Promoting Rhizobacteria (PGPR) mediated resistance”. PhD thesis entitled on “Exploring the mulberry endophytes for the management of mulberry root rot disease”.
Mulberry, a multipurpose tree known as Kalpavriksha, suffers severe leaf yield loss due to root rot disease caused by Macrophomina phaseolina. The pathogen was isolated and confirmed based on cultural, morphological, and microscopic characteristics including sclerotial formation. A total of 105 endophytes comprising bacteria, fungi, and actinobacteria were isolated from healthy mulberry plants, predominantly from root tissues. In vitro antagonistic screening identified highly effective bacterial (ENB1), fungal (ENF20), and actinobacterial (ENA4) endophytes against the pathogen. Selected endophytes exhibited important biochemical traits such as enzyme production, siderophore formation, and IAA production. Molecular identification revealed key antagonists as Bacillus methylotrophicus, Trichoderma asperellum, and Streptomyces rochei. Pot culture experiments demonstrated improved plant growth and higher survival rates in endophyte-treated mulberry plants. Combined application of B. methylotrophicus, T. asperellum, and S. rochei showed synergistic suppression of root rot with up to 75% survivability. SEM studies confirmed severe morphological damage to M. phaseolina hyphae due to endophyte interaction. Vermicompost-based consortia formulation enhanced endophyte viability, induced systemic resistance, and proved most effective under in vivo conditions.
| Publication | Number |
|---|---|
| Full length Research Papers | 4 |
| Research Short notes | - |
| Books | - |
| Book chapters | 12 |
| Research abstracts | 1 |
| Popular articles | 11 |
| TV/Radio Programmes | - |